Plasmid

Part:BBa_K4231017

Designed by: Shi Han   Group: iGEM22_USTC   (2022-09-30)


pUC19 carrying with ERG10, ERG13 and URA3 tag from Y.lipolytica

Using pUC19 as plasmid backbone, AmpR as reporter gene in bacteria, URA3 tag as reporter gene in Yarrowia lipolytica, shuttle plasmid carrying ERG10 and ERG13 from Yarrowia lipolytica. The plasmid can be effectively amplified in E.coli DH5a and can be successfully expressed in Yarrowia lipolytica. At the same time, ERG10 and ERG13 are important enzyme genes in the MVA pathway, and they carry the PTS1 sequence to help the enzyme protein locate in the peroxisome. Therefore, the plasmid plays an important role in the construction of MVA pathway in the peroxisome of Yarrowia lipolytica.

PUC-ERG10-ERG13-URA3.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2741
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1391
    Illegal XhoI site found at 2737
    Illegal XhoI site found at 2779
    Illegal XhoI site found at 4247
    Illegal XhoI site found at 4280
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 634
    Illegal PstI site found at 3745
    Illegal NgoMIV site found at 8
    Illegal NgoMIV site found at 889
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 3187
    Illegal NgoMIV site found at 4435
    Illegal AgeI site found at 3673
    Illegal AgeI site found at 4538
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1415
    Illegal BsaI site found at 2140
    Illegal SapI site found at 1248


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Categories
Parameters
None